I would highly recommend Dr. Sylvia Janetzki and ZellNet Consulting, Inc., for the development or improvement of ELISPOT assays. During an ELISPOT reader workshop, Sylvia personalized our training which included setting up parameters for our individual experiments saving us a lot of time. She has been available every time we have needed to reach her to troubleshoot issues we've encountered and she has resolved them quickly. In a second workshop, she provided us with invaluable information which has not only improved the quality of our ELISPOT assays, but also our work with human PBMCs in general. We look forward to working with ZellNet Consulting, Inc. in the future.