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    Services

Zellnet Consulting, Inc.  
(201) 346-0710  

info@zellnet.com  




Evaluation - Solutions - Training

ZellNet Consulting offers a wide range of services to help you establish and improve the Elispot technique in your laboratory.






Automated Elispot Reader System

Evaluation
Reading of Elispot Plates including mutiple color plates

Your plates will be evaluated in high resolution (pixel size <5μm) using an automated Elispot reader system (Carl Zeiss) with KS Elispot Software 4.9. You will receive the following information:
  • number of spots per well
  • size of each spot in each well
  • intensity of each spot in each well
  • total area per well covered by spots
  • plate overview image
  • binary spot picture of all wells
  • single well images
  • reader settings

Provided well images can be used for publication, demonstration and presentation purposes. Per request, we can also provide high resolution well images for re-evaluation purposes

Evaluation costs for single color plates: $ 33.00 per plate

For express service (by request - in 24 hours), an additional fee of $17.00 per plate applies.

Dual-color plates (enzymatic or fluorescent staining) will be read 3x (e.g. for enzymatic: red, blue and purple (=double-stained) spots).
Price: $ 60.00 per plate (non-fluorescent)
$75.00 for dual stained fluorescent plates

Shipping: $5.00 per package - independent of number of plates

Average turn-around time: 2-3 days







Evaluation
Membrane Removal

For highest accuracy, we strongly recommend to remove the membranes from Elispot plates and transfer them onto sealing tape. This assures that membranes are completely flat and therefore perfectly focussed during evaluation (Most plates give membranes room to expand. This leads to concave membranes, with their center being elevated in comparison to the well periphery.). Membrane removal further avoids any light reflection from well walls. In addition, it offers a convenient storage solution.

ZellNet offers membrane removal service for a nominal fee ($9.00 per plate), or an easy to use membrane removal device.







Evaluation
Certification Service

Operator-dependent variability of Elispot reading results is a known phenomenon, and has been described. ZellNet offers a service to certify proficiency in Elispot plate evaluation. This should be periodically repeated in each lab running their own plate evaluation. This service is independent of the Elispot reader you are using in house.

Simply send your plate(s) in for re-evaluation. Specify the request for certification. Also, provide your reading results and, if feasible, setup information about your plate(s). We will perform an extensive auditing of all wells, spot appearance,analysis of artifacts or any problems observed (if necessary) and provide an extensive statistical analysis and comparison.

Please, inquire for more details and pricing.







Solutions
Consulting

Our consulting services cover any aspect of the Elispot, including trouble shooting, review of protocol, SOP writing, standardization and validation procedures and guidelines, setup for clinical trial monitoring, certification services, evaluation of plates, training procedures, setup and performance of proficiency panels, GLP compliance and more.









Training
Seminars

You can hire one of our scientists to give a seminar at your location. You choose the topic, we do the rest. Examples of seminars are:
  • Principles of the Elispot technique
  • Standardization and validation procedures
  • Elispot and GLP
  • New Elispot applications and techniques
  • Troubleshooting
  • Elispot market and fields of application
  • Evaluation of Elispot plates

Travel and accommodation charges will be added to the consulting fee, if applicable.







Training
Workshops

This is a training for groups of 1 - 10 people*. The goal of our workshops is to provide technical expertise:
  • to perform an Elispot assay
  • for cell preparation including PBMC isolation, freezing and thawing procedures
  • isolation of cell subpopulations
  • cell counting
  • Elispot plate evaluation
Workshops include
  • establishment of lab-specific protocol
  • hands-on training for Elispot performance
  • peformance of complete Elispot experiment by participants using lab-specific protocol
  • troubleshooting of typical problems encountered
  • discussion of performed experiment and results
  • strategy setup for standardization and validation procedures as well as training guidelines

*: Please, note that the training is typically performed in your laboratory/facilities. Travel and accommodation charges will be added to the workshop fee.



















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