Caution!

Visiting this web site requires a newer version of Netscape Communicator.

Visit Microsoft's Web site to obtain the newest version of Internet Explorer, or visit Netscape's Web site to obtain the newest version of Netscape Communicator.

Visiting this web site without first upgrading your browser may result in unreliable behavior.











About ZellNet



Home


Contact Information


Testimonials


Services


ELI-Puncher Kit


FAQ

Additional Resources



Protocols


Related Links

Interactive



Elispot Discussion


Career Corner

Newsletter



Validation project


CVC EPP announcement


Elispot plates column


Perforin Elispot


Fluorospot


Synthetic peptides


Dual Color Elispot

Workshops



Duke - Validation


Elispot Resource Group


Registration Milano


Registration Rome


Sitemap




    Protocols

Zellnet Consulting, Inc.  
(201) 346-0710  

info@zellnet.com  




Please, find below some commonly requested protocol recommendations.





AEC preparation
  1. Add 1 tablet of AEC (Sigma) to 2.5 ml dimethylformamid (in 50 ml tube). Wait until completely dissolved (app. 5 minutes).
  2. In the meantime, prepare acetate buffer as follows:
    • 46.9 ml dd H2O
    • 4.6 ml 0.1 N acetic acid
    • 11 ml 0.1 M sodium acetate
  3. Add 47.5 ml of acetate buffer to dissolved AEC tablet.
  4. Add 25 ul 30% H2O2 and mix well.
  5. Filter through 0.45 um filter.
  6. Add 100 ul AEC solution per well.

















Sign In
Sign In