ZellNet's services are available world-wide. Please, contact us for specific information concerning your region.

Our turn-around time is in average 2-3 days. Express service for evaluation in 24 hours is also available. For more information, please check our service section.

Yes, we are always trying to help you improve your Elispot assay performance. If we observe problems in your Elispot assay during the evaluation of plates, we will provide you with a range of solutions to solve them. This service is free of charge.

We are using the high resolution automated Elispot reader system from Carl Zeiss with its newest KS Elispot software version.

There are in general 2 different reader systems out there. Most reader systems take one picture per well. The resolution of the picture taken depends therefore only on the camera used, and can be expressed in the pixel size (smallest unit of a picture). With one picture per well, the average pixel size is 15 - 35 µm. This should be referred to as low resolution in Elispot evaluation. With a high resolution system, multiple pictures per well are taken. The resolution is therefore not only dependent on the camera, but also on the magnifying system used (microscope). The average pixel size is 2 µm,and the resolution is multiple times higher than in a low resolution system.

An accurate image analysis requires at least 7 pixels (smallest unit of a picture) across the object to be analyzed. The average spot size (= object) is 20 -100 µm in diameter. With a high resolution reader (pixel size = 2 µm), this translates into 10 - 50 pixels across the spot. An accurate analysis of a spot including the recognition and exclusion of artifacts is possible. A low resolution system (pixel size in average 15 µm) can only provide 1 - 7 pixels across the spot. Small spots and artifacts appear as rectangles and look very much alike. No accurate evaluation can be assured.

The resolution of readers taking one picture per well depends on the camera, especially their chip size which determines the average pixel size of the picture of one well of a 96 well plate. The most sophisticated analog cameras are able to reach a pixel size of 9 µm. Most readers use cameras offering a pixel size of 15 - 35 µm. Keeping in mind that 7 pixels are needed across the diameter of a spot for accurate evaluation, only spots larger than 7 x 9 µm = 63 µm are evaluated accurately. High end digital cameras are able to slightly decrease the pixel size. However, they increase the evaluation time and are very expensive. For Elispot evaluation purposes, pixel sizes reached with systems taking one picture per well should be referred to as low resolution systems. Objects (spots, artifacts) below 60 µm in diameter can't be analyzed precisely.

Yes. The KS Elispot software allows us to adapt the evaluation parameters for specific spot features including the color range. Double stained plates are evaluated for 3 color ranges (typically red, blue and purple for double stained spots).

Yes. The reader system is able to evaluate fluorescent Elispot samples as well.

In general, yes. Scanning services do not include the actual evaluation of your wells. ZellNet offers an evaluation service that includes scanning by default. This means, that all wells are scanned and evaluated at the same time. There is no additional time required for evaluation after scanning the wells. However, scanned pictures can be stored and re-evaluated, if needed.

No, there is no software requirement. ZellNet's service includes scanning and evaluation. You will get multiple data and picture files from the evaluation of your plate.

No, there is no plate requirement. Any type of plates used for Elispot assays can be evaluated by us.

Yes, we are able to evaluate clear plastic plates. There is no restriction on the plates which can be sent for evaluation.

Yes, we are able to evaluate gold stained Elispot plates.

No. ZellNet is an independent consulting company. ZellNet does not sell any antibodies, plates or similar items to be used in the Elispot assay. For the availability of the Eli-Puncher kit for membrane removal, please check here.