Elispot Discussion

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Elispot Discussion

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Subject Granzyme B Elispot

Date Wed Oct 29 2008 13:32

Author Mike (mikec@zellnet.com)



Depends on how large your apc are. If they are large tumor cells or similar, 30,000 cells per well sounds perfect. I recommend plating out effectors first (100,000 is a good number), let them settle for 1-2 hours, then add apc carefully. PHA is an excellent choice.

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