Elispot Discussion

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Elispot Discussion

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Subject Coating with peptide

Date Wed Dec 19 2007 16:49

Author Beth Vorderstrasse (vordersb@wsu.edu)



I am new to the elispot assay and am trying to use the technique to identify influenza peptide-specific AFC. We would like to coat the plates with the peptide instead of a primary antibody, and then use isotype-specfic antibodies to identify different AFC populations. Is there any reason to think that coating plates with peptide would be any different than with antibody? Should I stick with the 10 ug/ml concentration as recommended in the Millipore protocol for the IFNg elispot?

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