Elispot Discussion

Caution!
Visiting this web site requires a newer version of Netscape Communicator.

Visit Microsoft's Web site to obtain the newest version of Internet Explorer, or visit Netscape's Web site to obtain the newest version of Netscape Communicator.

Visiting this web site without first upgrading your browser may result in unreliable behavior.



	About ZellNet

		Home
		Contact Information
		Testimonials
		Services
		ELI-Puncher Kit
		FAQ
	Additional Resources

		Protocols
		Related Links
	Interactive

		Elispot Discussion
		Career Corner
	Newsletter

		Validation project
		CVC EPP announcement
		Elispot plates column
		Perforin Elispot
		Fluorospot
		Synthetic peptides
		Dual Color Elispot
	Workshops

		Duke - Validation
		Elispot Resource Group
		Registration Milano
		Registration Rome

		Sitemap

Elispot Discussion

Zellnet Consulting, Inc.
(201) 346-0710

info@zellnet.com

Elispot Discussion: Please, feel free to start discussions about the Elispot technique, methods, ideas, questions, experience etc. With your input, we would like to make this website to a special interactive place for everyone who is interested in the Elispot assay.

We apologize for not having this discussion site online for a while. Our web site was targeted by someone who placed a bad activeX link in the discussion. We are in the process of recovering as much as possible. The shown placement dates do not reflect the actual message posting. The first discussion topic was placed in 1999.

Subject murine Elispot

Date Wed Dec 19 2007 16:14

Author Christie (doxse001@umn.edu)



I have been trying to develop a protocol for doing mouse ELiSPOTs, specifically to detect mouse IL-2 and mouse IFN-g. So far, I have had absolutely NO LUCK. I am using antibodies created for doing ELISAs from PharMingen and have attempted to titrate them. The problem is that my negative and positive (ie "non-immunized" and "immunized" mouse spleen stimulated with Con A) samples look identical (lots of REAL looking spots). All of my incubations and washes are the same as a neighboring lab whose human ELiSPOTS seem to work beautifully. I am suspecting the problem is in the preparation of the cells. Could the problem be using the whole spleen or in the stimulation (I know in human they use purified cell populations)? Has ANYONE out there done a mouse ELiSPOT that works??!?! Please help!!! Thanks.

Click here for this discussion's index