Elispot Discussion

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Elispot Discussion

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Subject Rings

Date

Author Sylvia (sylvia@zellnet.com)



Esther, those rings typically occur when some substrate is left in the plates. That happens if the underdrain (this plastic basin on the bottom of the IP or HA plate) is left on the plate after stopping the spot development. Please, remove if as soon as you stop the color development, remove all remaining liquid from the plate and dry the wells by hand with a paper towl. As longer you do that, as more liquid is absorbed from the wells, and finally you should not have any rings left as you descibed. Just to summarize, those are from the substrate left in the underdrain. Hope this helps. Sylvia .

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