Elispot Discussion

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Elispot Discussion

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Subject blocking

Date Wed Nov 14 2007 18:37

Author Sylvia (sylvia@zellnet.com)



Hi! In my experience, it takes a while to optimize the protocol for your needs. Blocking with PBS/BSA should be fine. However, you can also use your RPMI + serum. If you get spots without adding cells (did I understand this right?) then this might be due to whatever you use for coating. Spots in wells without cells can also be due to the FCS you are using or some infection in development buffers. Which method do you use? Avidine likes to bind to many things, also bacterial proteins from special strains - this can cause "beautiful" spots.

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