Elispot Discussion

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Elispot Discussion

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Subject GrB versus IFN-g

Date Wed Nov 14 2007 18:21

Author Kimberly Shafer-Weaver (Shafer_Weaver_K@ncifcrf.gov)



Jeff, You might want to check out our paper. The Granzyme B ELISPOT assay: an alternative to the 51Cr-release assay for monitoring cell-mediated cytotoxicity Kimberly Shafer-Weaver, Thomas Sayers, Susan Strobl, Eric Derby, Tracy Ulderich, Michael Baseler, Anatoli Malyguine Journal of Translational Medicine 2003, 1:14 (29 December 2003) It is not unusual to see differences between the number of GrB and IFN-g spots. One reason is that not all CTL that secrete GrB also secrete IFN-g. It could also be a function of your stimulant. We have found that different peptides can elicit differnt types and manitudes of response. Additionally, it seems that peptides with high binding avidity/affinity elicit a better GrB response.

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