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    Elispot Discussion

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Elispot Discussion

Please, feel free to start discussions about the Elispot technique, methods, ideas, questions, experience etc. With your input, we would like to make this website to a special interactive place for everyone who is interested in the Elispot assay.


We apologize for not having this discussion site online for a while. Our web site was targeted by someone who placed a bad activeX link in the discussion. We are in the process of recovering as much as possible. The shown placement dates do not reflect the actual message posting. The first discussion topic was placed in 1999.


Subject GrB

Date Wed Nov 14 2007 18:19
Author David Baloyan (davidbaloyan@yahoo.com)

Dear Dr.Voorn
Disturbing you from Georgian AIDS Research Center. We want to set up CTL assay in our laboratory. Would you be so kind to tell us if Elispot method as sensitive as flow cytometry CTL functional method (Using detection of intracellural GrB in T cells and 7AAD on target cells ).Second queston: Does analizing of Elispot plates using light microscope be as informative as by Elispot reader,because for present time we have no possibilities to buy Ellispot reader. Will be such evaluation method informative or not.
Thank you.
With respect David Baloyan

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