Elispot Discussion

Caution!
Visiting this web site requires a newer version of Netscape Communicator.

Visit Microsoft's Web site to obtain the newest version of Internet Explorer, or visit Netscape's Web site to obtain the newest version of Netscape Communicator.

Visiting this web site without first upgrading your browser may result in unreliable behavior.



	About ZellNet

		Home
		Contact Information
		Testimonials
		Services
		ELI-Puncher Kit
		FAQ
	Additional Resources

		Protocols
		Related Links
	Interactive

		Elispot Discussion
		Career Corner
	Newsletter

		Validation project
		CVC EPP announcement
		Elispot plates column
		Perforin Elispot
		Fluorospot
		Synthetic peptides
		Dual Color Elispot
	Workshops

		Duke - Validation
		Elispot Resource Group
		Registration Milano
		Registration Rome

		Sitemap

Elispot Discussion

Zellnet Consulting, Inc.
(201) 346-0710

info@zellnet.com

Elispot Discussion: Please, feel free to start discussions about the Elispot technique, methods, ideas, questions, experience etc. With your input, we would like to make this website to a special interactive place for everyone who is interested in the Elispot assay.

We apologize for not having this discussion site online for a while. Our web site was targeted by someone who placed a bad activeX link in the discussion. We are in the process of recovering as much as possible. The shown placement dates do not reflect the actual message posting. The first discussion topic was placed in 1999.

Subject GrB

Date Wed Nov 14 2007 18:18

Author John Voorn (j.voorn@sanquin.nl)



The mystery of the granzyme B antibodies is actually quit simple; there is only one antibody-pair suitable for ELISPOT, clones GB10 and GB11. These antibodies have been made by Sanquin (former CLB) in Amsterdam. (first publication already published in 1993 in J Immunol Methods. Jul 6;163(1):77-83. Production and characterization of monoclonal antibodies raised against recombinant human granzymes A and B .... Kummer JA, et all.) All companies selling granzyme B ELISPOT kits, pairs etc. have a licence from Sanquin. Next to this Sanquin it selves also sells these antibodies as well as a dedicated kit for ELISPOT, see www.sanquinreagents.com

Click here for this discussion's index