Elispot Discussion

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Elispot Discussion

Zellnet Consulting, Inc.
(201) 346-0710

info@zellnet.com

Elispot Discussion: Please, feel free to start discussions about the Elispot technique, methods, ideas, questions, experience etc. With your input, we would like to make this website to a special interactive place for everyone who is interested in the Elispot assay.

We apologize for not having this discussion site online for a while. Our web site was targeted by someone who placed a bad activeX link in the discussion. We are in the process of recovering as much as possible. The shown placement dates do not reflect the actual message posting. The first discussion topic was placed in 1999.

Subject	Date	Author


monkey Elispot	Wed Nov 14 2007 18:04	Peter
��cynomolguns Elispot	Tue Feb 17 2009 15:11	Milton
��monkey Elispot	Wed Nov 14 2007 18:09	Chris Miller
��monkey	Wed Nov 14 2007 18:06	Devish
��monkey Elispot	Wed Nov 14 2007 18:08	Sylvia
��monkey Elispot	Wed Nov 14 2007 18:11	Carol
��monkey Elispot	Wed Nov 14 2007 18:04	Sarah Stark
GranzymeB	Wed Nov 14 2007 18:12	Jeff Currier
��GrB	Wed Nov 14 2007 18:23	Sylvia
��Granzyme B elispot	Fri Sep 12 2008 12:39	Arun
��Granzyme B Elispot	Wed Oct 29 2008 13:32	Mike
��GrB	Wed Nov 14 2007 18:24	Jeff Currier
��GrB	Wed Nov 14 2007 18:25	Maryke Reed
��GrB	Wed Nov 14 2007 18:26	Sara Seaborn
��GrB versus IFN-g	Wed Nov 14 2007 18:21	Kimberly Shafer-Weaver
��GrB	Wed Nov 14 2007 18:16	Sep
��GrB	Wed Nov 14 2007 18:18	John Voorn
��GrB	Wed Nov 14 2007 18:19	David Baloyan
��GrB	Wed Nov 14 2007 18:14	David Baloyan
��GrB evaluation	Wed Nov 14 2007 18:15	Mike
no cell control	Wed Nov 14 2007 18:27	Ed
��background	Wed Nov 14 2007 18:29	Cathie from France
��false positive spots	Wed Nov 14 2007 18:31	Sylvia
��filter	Wed Nov 14 2007 18:33	Shai
��filter	Wed Nov 14 2007 18:33	Sylvia
��background	Wed Nov 14 2007 18:28	Terry Guthrie
Blocking	Wed Nov 14 2007 18:35	Retro
��blocking	Wed Nov 14 2007 18:37	Sylvia
��Blocking	Wed Nov 14 2007 18:36	Ajit
Rings		Carol
��Rings		Peter Mellings
��Rings		Esther
��Rings		Sylvia
Substrate		Karen Smith
��Substrate		Mike
Disturbing background spots		Jeff Currier
��Background spots		Sylvia
Plate washing	Wed Nov 21 2007 14:46	Cathy T
��Plate washer	Wed Nov 21 2007 15:04	Carol
��Plate washer	Wed Nov 21 2007 15:03	Pat
��Plate washer	Wed Nov 21 2007 14:51	Mike
��Plate washer	Wed Nov 21 2007 14:49	Carol B.
��Plate washer	Wed Nov 21 2007 14:48	Terrie Guthrie
peptide pool concentration	Wed Nov 21 2007 15:05	Clement Wesley
��peptide pool concentration	Wed Nov 21 2007 15:07	Cindy
��peptide pool concentration	Wed Nov 21 2007 15:08	Sylvia
Imaging punched membranes	Wed Nov 21 2007 15:09	Cathy
��Imaging punched membranes	Wed Nov 21 2007 15:10	Sylvia
��removing poster spray from the insert	Tue Nov 27 2007 10:32	kathy
��removing poster spray from stage insert	Mon Dec 31 2007 14:01	Mike
murine Elispot	Wed Dec 19 2007 16:14	Christie
��Murine Elispot	Wed Dec 9 2009 10:52	Leah
��murine Elispot antibodies	Wed Dec 19 2007 16:28	Sylvia
��murine Elispot	Wed Dec 19 2007 16:15	Christina
��murine Elispot	Wed Dec 19 2007 16:17	Jacqueline
Membrane binding	Wed Dec 19 2007 16:30	Kendra
��Membrane binding	Wed Dec 19 2007 16:33	Sylvia
White spots	Wed Dec 19 2007 16:36	Helena
��White spots - Biosource kit	Wed Dec 19 2007 16:43	Melanie
��Black spots	Wed Dec 19 2007 16:45	Hellfyre
��Black ELISPOT	Mon Jan 28 2008 12:13	Maya
��black Elispot	Mon Mar 10 2008 21:39	Mike
Peptides	Wed Dec 19 2007 16:46	Carol
��Peptides	Wed Dec 19 2007 16:48	Alefia
Coating with peptide	Wed Dec 19 2007 16:49	Beth Vorderstrasse
��Coating with peptide	Wed Dec 19 2007 16:51	Peter Mellings
��B cell isotype production	Wed Dec 19 2007 16:52	Beth Vorderstrasse
��coating with peptide	Wed Dec 19 2007 16:55	Mike
which plates to use	Wed Dec 19 2007 16:58	Michael
��plates	Wed Dec 19 2007 17:05	Peter Mellings
��tried plates	Wed Dec 19 2007 17:06	Michael
��plate cat#	Wed Dec 19 2007 17:07	SHAI
��plates	Wed Dec 19 2007 17:09	Michael
��plates	Wed Dec 19 2007 17:04	Melanie
Crescent-like development of well	Wed Dec 19 2007 17:11	Kim Z
��spot distribution	Wed Dec 19 2007 17:12	Sylvia
��spot distribution	Wed Dec 19 2007 17:13	Kim Z
��spot distribution	Wed Dec 19 2007 17:14	Sylvia
��target cells	Wed Dec 19 2007 17:20	Svetlana
��pulsing target cells	Wed Dec 19 2007 17:21	Sylvia
Th1/Th2 profile in post BMT patients	Wed Dec 19 2007 17:23	Raphael Rousseau
removal of old spray mount	Wed Dec 19 2007 17:25	Peter hayes
��removal of spray	Wed Dec 19 2007 17:26	Sylvia
IFNg background	Wed Dec 19 2007 17:42	Stacey Lapp
��IFNg background	Wed Dec 19 2007 17:43	Raphael Rousseau
��IFNg backgorund spots	Wed Dec 19 2007 17:47	Lugos Moses
��background spots	Wed Dec 19 2007 17:50	Peter Mellings
��filtering of secondary ab	Wed Dec 19 2007 17:51	Cathy T
��Filtering secondary ab	Wed Dec 19 2007 17:53	Sylvia
diffuse spots on IFNg ELISPOT with HA	Wed Dec 19 2007 17:55	Evan Serfass
��diffused spots	Wed Dec 19 2007 17:57	Peter
background spots all over, except the no	Wed Dec 19 2007 17:58	Tony
false negative results	Wed Dec 19 2007 18:02	Rosemarie Mason
��false negative results	Wed Dec 19 2007 18:03	Mike
Granzyme B Elispot	Wed Dec 19 2007 18:06	martin marek
��Granzyme B protocol	Wed Dec 19 2007 18:08	John Voorn
Storage of plates without loosing spots	Wed Dec 19 2007 18:09	Gmerice Hammond
��plate storage	Wed Dec 19 2007 18:10	Sylvia
��plate storage	Wed Dec 19 2007 18:11	Tony
��plate storage	Wed Dec 19 2007 18:12	Sylvia
Using protein instead of peptide	Wed Dec 19 2007 18:15	Rob Moss
��protein vs.peptide	Wed Dec 19 2007 18:16	Peter Mellings
incubation with primary antibody	Wed Dec 19 2007 18:19	kamlesh
��coating temp	Wed Dec 19 2007 18:20	Sylvia
CEF peptide pool	Wed Jan 16 2008 15:18	Vijaya
Background spots	Thu Jan 24 2008 19:52	Alefia
��Background spots	Thu Jan 24 2008 19:54	Sylvia
Mesh-like IFNg-background	Thu Jan 24 2008 19:56	Eli Boritz
Monoclonal vs. Polyclonal	Thu Jan 24 2008 19:58	Kris
Elispot for antibody-forming B cell,help	Thu Jan 24 2008 20:00	luo ying
rapid membrane removal device	Thu Jan 24 2008 20:01	Geff Cole
blood storage in fridge?	Thu Jan 24 2008 20:02	adz
��cells in fridge	Thu Jan 24 2008 20:07	Peter Mellings
��blood storage	Thu Jan 24 2008 20:03	Sylvia
background spots	Thu Jan 24 2008 20:08	Catherine from France
��backgorund spots	Thu Jan 24 2008 20:09	Sylvia
��background spots	Thu Feb 28 2008 10:00	Dawn Mueller
how to describe spot size?	Thu Jan 24 2008 20:11	Laurie
pH of coating buffer	Thu Jan 24 2008 20:12	Karl Karlsen
��pH of coatiing buffer	Thu Jan 24 2008 20:13	Mike
DAB and HRP	Thu Jan 24 2008 20:14	Karl Karlsen
��DAB and HRP	Thu Jan 24 2008 20:15	Mike
IFNg kinetics + spots from unstim. cells	Thu Jan 24 2008 20:16	Anna-Karin
��less cells and short incubation	Thu Jan 24 2008 20:17	bongsu
Elispot reader	Thu Jan 24 2008 20:19	Susanne Ebner
��Elispot Reader	Mon Jun 22 2009 17:09	Dr. Nibhriti Das
��Elispot reader	Thu Jan 24 2008 20:20	Sylvia
��Elispot reader	Thu Jan 24 2008 20:24	Susanne
��Elispot reader	Thu Jan 24 2008 20:25	Sylvia
��Elispot reader	Thu Jan 24 2008 20:38	John Voorn
��Elispot reader	Thu Jan 24 2008 20:34	Sylvia
How to count spots?	Thu Jan 24 2008 20:39	Yifan
��counting spots	Thu Jan 24 2008 20:40	Mike
��Counting Ig spots	Wed Apr 9 2008 14:38	Ellie
��IgM spot image	Thu Apr 17 2008 17:29	Mike
Rat IL4 Elispot - help	Thu Jan 24 2008 20:41	Shai
��rat IL4 Elispot	Thu Jan 24 2008 20:42	Sylvia
��ConA for IL4	Thu Jan 24 2008 20:44	Shai
��ConA for IL4	Thu Jan 24 2008 20:44	Sylvia
Optimal cell numbers & peptide concentr	Thu Jan 24 2008 20:46	Sheila
��Peptide concentrations	Thu Oct 15 2009 11:21	Sam
��cells and peptide	Thu Jan 24 2008 20:47	Sylvia
BKV Elispot	Wed Feb 20 2008 22:36	Alexander Prosp
��BKV Elispot	Mon Mar 10 2008 21:44	Mike
Protein-pulsed APCs	Thu Mar 20 2008 09:15	Jessica
��whole protein ELISPOT	Wed Oct 29 2008 11:48	Nazzarena
Dual Color Antibody Detection	Fri Mar 21 2008 16:27	Jakub Simon
��Dual Color Antibody Detection	Mon Sep 1 2008 16:31	Jakub Simon
clear or white plate	Tue May 6 2008 14:49	diane rottembourg
��plate choices	Wed May 28 2008 15:01	Sylvia
Antibody secreting cells	Fri May 9 2008 16:27	Barbara Stewart
��Ab secreting cells	Tue Jun 3 2008 10:55	Sylvia
general info on elispot	Thu Jun 19 2008 21:40	Omer
��general info	Tue Jul 8 2008 13:39	Mike
general info on elispot	Thu Jun 19 2008 21:40	Omer
Isolation of PBMC	Wed Jul 9 2008 13:40	Angela
Choice of ELIspot plate : clear or white	Sun Oct 12 2008 22:24	Lee HyunKyoung
��Choice of plate	Wed Oct 29 2008 13:27	Mike
ELISPOT and BL3	Sat Jan 10 2009 10:37	Jackie Gillis
Streptavidin	Wed Jan 14 2009 10:16	Ishac
��Streptavidin	Sun Apr 5 2009 16:27	Mike
Interpreting the results	Thu Jan 29 2009 13:59	Damian
��Elispot reader	Mon Jun 22 2009 17:18	Dr. Nibhriti Das
TT Elispot	Thu Apr 16 2009 16:59	Nancy
no primary ab	Sun Jun 21 2009 12:32	Katja
��false positive spots	Wed Aug 19 2009 17:35	Sylvia Janetzki
Elispot Inf-gamma cynomolgus monkeys	Thu Nov 12 2009 13:33	Brouwers
IFN-g Murine Background	Wed Dec 9 2009 10:59	Leah
��IFN-g Murine Background	Thu Mar 4 2010 12:58	Mike Coland